Fig. 3. HSF2 and β-gal expression in the ventricular zone of embryonic and adult brain. (A) Parasagittal section of an E13.5 Hsf2^–/– embryonic brain showing β-gal expression along the lumen of the ventricles. fb, forebrain; mb, midbrain; chp, choroid plexus. (B and C) HSF2 immunolocalization and BrdU staining, respectively, at the level of midbrain in E12.5 Hsf2^+/+ embryos. vz, ventricular zone; lv, lumen of the ventricle. (D–F) β-gal detection in the ependymal layer of adult Hsf2^–/– brain. st, striatum; se, septum; ep, ependymal zone; lv, lateral ventricle. Scale bar: 50 µm. (E) Detail of (D), magnification: 20×. (F) Detail of a transverse section at the level of the hippocampus (h), magnification: 20×.