Table I. PCR primers.

	Gene or RNA	PCR primers	PCR product
Southern blotprobe	HSF2 (exon 4)	5′-gcgGTTTCATCTTCAAAACCAGAGG-3′5′-TCTGAAAGCCTGGACTCAATAG-3′	119 bp
PCR forgenotyping	Primer 1[end of intron preceding exon 5;Manuel et al. (1999)–beginning of exon 5 nucleotides614–636 of the cDNA; Sarge et al. (1991)]	5′-GAGTGAGAATGAATCCCTTTGGAAGG-3′
	Primer 2	5′-GCTGGAAGCTTCTTACCTTCCG-3′	95 bp
	(end of exon 5, nucleotides 685–689 and16 first nucleotides of the following intron;reverse orientation)
	Primer 3	5′-ATGTGCTGCAAGGCGATTAAGTTGG-3′	132 bp
	[beginning of the lacZ portion of the β-geo gene;Kalnins et al. (1983)]
RT–PCR	LH receptor	5′-CTTATACATAACCACCATACCAG-3′	517 bp
		5′-ATCCCAGCCACTGAGTTCATTC-3′
	HSF2	5′-ATCCCTTTGGAAGGAGGTGT-3′	114 bp
		5′-TCACAAGTTGATTATTCTGAACCAA-3′
	Recombinant chimeric β-geo	5′-ATCCCTTTGGAAGGAGGTGT-3′	290 bp
		5′-GACAGTATCGGCCTCAGGAA-3′
	GAPDH	Xu et al. (1999)	300 bp
	Actin	5′-TGTTACCAACTGGGACGACA-3′	396 bp
		5′-CTCTCAGCTGTGGTGAA-3′