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. 2005 Oct 7;33(18):e151. doi: 10.1093/nar/gni144

Figure 1.

Figure 1

Design and application of E7 crook siRNA for use in quantitative PCR. (a) Sequence and schematic representation of E7 crook siRNA. (b) Design of the crook template and positions of upstream (E7 crook siRNA) and downstream primers (crook dn7 and crook dn5) for PCR amplification. The crook template (312 bp) is derived from the CMV promoter and is cloned into pBlueScript (pBS) to give pBSCrook (Materials and Methods). (c) Visualization of crook template DNA after PCR amplification using E7 crook siRNA and crook dn 7 as primers and agarose gel electrophoresis. E7 crook siRNA was added in a limiting dilution series, as indicated; crook template and crook dn7 primer were present in excess for the reaction (see text). (d) Read-out from quantitative PCR showing effects of E7 crook siRNA dilution series (333.3 to 5.2 nM) on product amplification and plateau levels (see text).