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. 2005 Oct 7;33(18):e151. doi: 10.1093/nar/gni144

Figure 2.

Figure 2

Recovery and quantification of E7 crook siRNA after transfection into human cells in culture. (a) Recovery of E7 crook siRNA duplex and also of single-stranded E7 crook siRNA sense strand at 24 and 72 h after transfection in to SiHa cells visualized indirectly by its ability to prime the amplification of the crook template. Parallel cell cultures were transfected with either duplex E7 crook siRNA or with single-stranded E7 crook sense RNA, as indicated. (b) Quantification of E7 crook siRNA (red triangles) using standard curves derived from a dilution series of E7 crook siRNA (Materials and Methods; internal standard dilution curves were performed for each experiment). E7 crook siRNA was recovered with total RNA 24 h after transfection into either SiHa or HCT116 cells, as indicated. (c) Recovery levels of E7 crook siRNA at 24, 48 and 72 h post-transfection into SiHa and HCT116 cells as indicated. (d) Detection of E7 crook siRNA from SiHa cells transfected with different input doses of the crook siRNA, as indicated.