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. 2025 Oct 21;53(19):gkaf1044. doi: 10.1093/nar/gkaf1044

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© The Author(s) 2025. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Spacer Duplication Correlates with Start Codon Enrichment. Spacers were grouped by duplication frequency, and the proportion in each bin containing an in-frame ATG was plotted. For example, the asterisk marks the 19-duplication bin, where 60% of spacers contained in-frame ATGs. Only 30 bp of each spacer could contribute an in-frame ATG due to overlap with the repeat. Assuming random composition, the probability of a 30 bp sequence containing an in-frame ATG lacking a downstream in-frame stop codon is 12% (dashed line and Supplementary Table S3).