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. 2025 Oct 21;53(19):gkaf1044. doi: 10.1093/nar/gkaf1044

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© The Author(s) 2025. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Transposon Mutagenesis Screen for Adaptation Regulators. (A) Workflow. Tn5 transpososomes carrying a chloramphenicol resistance marker and R6K origin were electroporated into RC5311. After selection, ∼15 000 colonies were pooled. The P_araBAD Cas1–Cas2 plasmid (pRC1656) was introduced, and adaptation was assessed using the papillation assay (0.002% l-arabinose). (B) Representative screening plate. Hypo-papillating mutants were identified visually. Insertion sites were mapped by plasmid rescue using the R6K origin and the chloramphenicol resistance marker. (C) Selected hypo-papillating mutants are shown.