Fig. 9. Enhanced inhibition of p53 transcriptional activity by Hdm2 F394. H1299 cells were transfected with expression plasmids for wild-type p53 (30 ng; black bar), either alone or together with expression plasmids for wild-type Hdm2 (400 ng; dark gray bar) or Hdm2 F394 (400 ng; light gray bar). The transcriptional activity of p53 was monitored by measuring the activity of the luciferase gene under the control of the cyclin G promoter (white bar). The results from three independent experiments are shown, each of which represents an average of three to seven independent transfections for each combination of expression plasmids. The standard error is given.