Table I. Percentage of photoaffinity labelling by 8-azido-[α-32P]ATP remaining after displacement with unlabelled ATP or ADP.
NBD1 (%) |
NBD2 (%) |
|||
---|---|---|---|---|
ATP | ADP | ATP | ADP | |
SUR1 | 12 ± 10 | 21 ± 14 | 52 ± 11 | 45 ± 11 |
SUR1-S830R | 8 ± 3.7 | 25 ± 9.0 | 43 ± 11 | 33 ± 6.8 |
SUR1-S1482R | 9 ± 5.1 | 21 ± 11 | 39 ± 14 | 34 ± 8.4 |
Values are expressed as a percentage of 8-azido-[α-32P]ATP bound in the absence of unlabelled ATP (100 µM) or ADP (100 µM). Experiments were performed in the presence of 1 mM Mg2+, as described in Materials and methods.