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. 2002 Aug 15;21(16):4250–4258. doi: 10.1093/emboj/cdf419

Table I. Percentage of photoaffinity labelling by 8-azido-[α-32P]ATP remaining after displacement with unlabelled ATP or ADP.

  NBD1 (%)
NBD2 (%)
  ATP ADP ATP ADP
SUR1 12 ± 10 21 ± 14 52 ± 11 45 ± 11
SUR1-S830R 8 ± 3.7 25 ± 9.0 43 ± 11 33 ± 6.8
SUR1-S1482R 9 ± 5.1 21 ± 11 39 ± 14 34 ± 8.4

Values are expressed as a percentage of 8-azido-[α-32P]ATP bound in the absence of unlabelled ATP (100 µM) or ADP (100 µM). Experiments were performed in the presence of 1 mM Mg2+, as described in Materials and methods.