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. 2025 Oct 8;16:1693135. doi: 10.3389/fpls.2025.1693135

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Copyright © 2025 Zhao, Xie, Han, Zhang, Qi, Zhang, Sun, Liu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Image A shows a series of Petri dish assays with yeast two-hybrid interactions. Each row corresponds to a different combination of vectors: pGADT7 with pGBKT7-PpGH17-8, pGADT7-PpKINβ2 with pGBKT7-PpGH17-8, and pGADT7-PpMIEL1 with pGBKT7-PpGH17-8. The Petri dishes show variations in blue color development under different growth media conditions labeled as -T/-L/-A/-H and -T/-L. Image B displays microscopic photographs of cells. The first row shows PpGH17-8-YN/YC with three grayscale images. The second row depicts PpGH17-8-YN/PpKINβ2-YC, presenting greyscale and yellow fluorescent images. The third row illustrates PpGH17-8-YN/PpMIEL1-YC with similar greyscale and fluorescent images. — PpGH17–8 interacts with PpKINβ2 and PpMIEL1. (A) Interaction between PpGH17-8, PpKINβ2 and PpMIEL1 in the Y2H assays. (B) Interaction between PpGH17-8, PpKINβ2 and PpMIEL1 in the BiFC assays.