Skip to main content
. 2025 Oct 21;11:117. doi: 10.1038/s41540-025-00590-2

Fig. 4. RNA-seq identifies gene expression clusters unique for specific patterns of JNK activation.

Fig. 4

A Schematic highlighting the time points where mRNA was collected. Data traces are mean traces from Fig. 2A–C used to highlight relationship of mRNA timepoints to JNK activity. B Principal Component Analysis (PCA) of RNA-seq timepoints and samples. C control, S sustained, T transient, P pulse. Numbers represent the specific time point. C–E K-means clusters of gene expression patterns for sustained (C), transient (D), or pulsed (E) JNK activity. Data shown as the mean ± SD of scaled expression data (0–1). N represents the number of genes within each cluster. F–H Number of JNK-dependent genes identified within sustained (F), transient (G), or pulsed (H) clusters.