Table I. Identification of genes that transcription specifically changed following the TIS7 overexpression by cDNA microarray analysis.
| Sequence annotation | UniGene accession No. | Differential expression: TIS7–EGFP | Real-Time PCR |
|---|---|---|---|
| Cellular retinoic acid binding protein II (crabp II)* | W81912 | –4.1 | –7.5 |
| Osteopontin/secreted phosphoprotein I (spp1)* | AA108928 | –2.9 | –2.1 |
| w.s.t. CG5676 gene product # | AA238637 | –2.6 | |
| h.s.t. AF150733_1 AD-014 protein # | W74865 | –2.2 | |
| Unknown EST | 34061 | –2.1 | |
| h.s.t. NADH-ubiquinone oxireductase B14 SU # | 62323 | –2.1 | |
| m.s.t. 60S ribosomal protein L39 # | AA178015 | –2.1 | |
| h.s.t. 60S ribosomal protein L22 # | AA174807 | –2.0 | |
| Unknown EST | W41563 | –2.0 | |
| Myosin heavy polypeptide 8, skeletal muscle, perinatal* | W13528 | –1.9 | |
| Synapsin Ib* | AA033398 | –1.9 | |
| w.s.t. wdnm1 protein precursor # | AA105830 | –1.8 | |
| Feminization-1a homolog (fem-1a)* | AA434902 | –1.8 | |
| h.s.t. cystathione-γ-lyase # | AA245993 | –1.7 | |
| Emopamil binding protein (ebp)* | W08348 | –1.7 | –2.8 |
| Cathepsin Z* | W14289 | –1.7 | |
| Peptidylprolyl-isomerase A* | AA499926 | –1.7 | |
| Fibroblast inducible secreted protein 12 (fisp12)* | W36541 | –1.7 | |
| Pituitary tumor transforming gene protein 1 (pttg1)* | A250500 | –1.6 | |
| Thymidine kinase 1* | AA041834 | –1.6 | |
| Unknown EST | W09957 | –1.6 | |
| Homeobox, msh-like 2* | AA016730 | –1.5 | |
| DNA segment, Chr 15, Wayne State University 77 | AA014127 | –1.5 | |
| Torsin family 2, member A* | AA190292 | –1.5 | |
| Hexokinase 1* | W11571 | –1.5 | |
| h.s.t. SRB7 # | AA067890 | –1.5 | |
| Split hand/foot deleted gene 1* | AA462396 | –1.5 | |
| h.s.t. ubiquinol–cytochrome c reductase complex (7.2 kDa) # | AA087137 | –1.5 | |
| h.s.t. myelin protein zero (p0) precursor # | AA097191 | +1.5 | +3.8 |
| Intracisternal A-particle mRNA | AA404014 | +1.5 |
Using the MicroarrayViewer program (see Supplementary data), genes specifically regulated by ectopic TIS7 could be extracted from the vector associated and from the great majority of unaffected genes. Candidate genes are listed according to their level of differential expression. w.s.t., weakly similar to; m.s.t., moderately similar to; h.s.t., highly similar to; *, functionally annotated TIS7 target genes; #, TIS7 target genes of ‘uncertain’ identity. Confirmation of the cDNA microarray hybridization results by Real-Time PCR analysis. Values of TIS7-regulated gene expressions were normalized and then compared as a ratio of TIS7 sample values and eGFP sample values.