Fig. 5. Effect of rACF on accessibility of damaged DNA in dinucleosomes. (A) The locations of the EaeI and RsaI sites on 5S dinucleosome DNA. Thick arrows show the location of the 5S RNA gene. Asterisks show the 6-4PP positions. (B) Restriction enzyme digestion of dinucleosomes. 5′-end-radiolabeled naked DNA (lanes 2 and 3) and dinucleosomes (lanes 4–12) were digested with either EaeI (top) or RsaI (bottom). The restriction digestion was carried out in the presence or absence of ACF (200 fmol) and 3 mM ATP as indicated at the top of each lane. Non, undamaged DNA; Center, center-dinucleosome DNA; Linker, linker-dinucleosome DNA. The percentage digestion of the 5′ EaeI and 5′ RsaI restriction sites is shown at the bottom of each lane.