Fig. 8. DREAM is phosphorylated by a PI 3-kinase-dependent pathway. FL5.12 cells were cultured in the presence of [³²P]ortho phosphate with or without LY294002. After labeling, cells were lysed and DREAM was immunoprecipitated using a specific polyclonal antibody and protein A/G–agarose beads. Phosphorylation of DREAM was determined by autoradiography.