Fig. 6. Effects of Smad3 and TGF-β on the function of CBFA1 and runt family transcription factors depend on the cell type and promoter. 10T1/2 cells (A and C) or HepG2 cells (B and D) were transiently transfected with the indicated expression plasmids and the luciferase reporter plasmids p6OSE2-Luc (A and B) or pCα179-Luc (C and D). Transactivation from these promoters was measured in the absence or presence of TGF-β (1 ng/ml). Luciferase expression from the reporter plasmids was scored as in Figure 2.