Fig. 4. Fluorescence level distributions in the autocatalytic systems. (A) Chromosomal reporter system. pBB240 was transformed into strains ABY001 (one copy of the reporter gene) and ABY011 (two copies of the reporter gene), and are represented by blue and green shades, respectively. Cells were grown in SD-Leu, Trp. (B) Intrinsic reporter system. Out of the series ABY021–040, ABY021 and ABY022 were examined with one and more than five copies of pAB247, and are represented by blue and yellow shades, respectively. Strains were grown in SD-Leu. pBB247 was transformed into GFY259-2B, grown in SD-Trp; results are represented by green shades. The black lane represents the galactose-regulated plasmid pBB407 (see Materials and methods).