Fig. 2. Inhibition of chemical cross-linking of Adx, Fln and CPR to P450MT2 by sequence-specific peptides. Cross-linking was carried out with unlabeled P450MT2 and ³⁵S-labeled Adx (A), Fln (B) or CPR (C) as described in Materials and methods, and the products were immunoprecipitated with P4501A1 antibody. The immunoprecipitates were resolved on 12% SDS–polyacrylamide gels and subjected to fluorography. The indicated amounts of MT2/I, MT2/II and MT2/III peptides were added before the addition of cross-linking agent, EDC. CLP = cross-linked product.