Table III. Host leader sequences in TSWV mRNAs.
Host gene | Obtained sequence of TSWV mRNA | No. of clones |
---|---|---|
ubi.U4 | 7mGpppAUCCUUUGAUUUCUCUA17UUCUC... | |
7mGppp-N16-A17GAGCAAUU... | 4 | |
SAM-DC | 7mGpppAUGGAGUCGAAAGGUG16GUAAAAAC... | |
7mGppp-N15-G16AGCAAUU... | 11 |
TSWV mRNAs primed by host mRNA leaders were amplified using nested RT–PCR. Individual clones from these amplifications were sequence analysed.