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. 2025 Oct 22;15:36934. doi: 10.1038/s41598-025-20920-y

Table 2.

Behavioural items recorded after the end of the procedure (Post MI). The score was obtained at multiple evaluations, starting from extubation until 12 h after sternal recumbency.

Appearance** Lying and restlessness
**
Tremors/ Spasms Vocalisation Food interest Agitation at
human approaching
*
Response to touch (neck, head, thorax and forelimbs)
*
0 Normal appearance Normal lying

No evidence

of tremors/

spasms

No vocalisation or vocalisation related to food/interest in human/mate approach Normal appetite Curious, interactive, may vocalise No response
1 Salivation Lying guarding one part of the body/moves without external stimulation Tremors/ spasms in one part of the body Vocalisation without a purpose/ unmotivated excitation Reduced appetite, eat special/ tasty food Moves away when approached Mild response: the animal looks uncomfortable and worried; retraction of the interested body part can be present
2 // Move often/ poor wake times

Generalised tremors/

spasms

// Reduced appetite independently of the food Biting and aggressive when approached Severe response: escape reaction
3 // Continuous pacing in the box // No appetite Stay immobile and disinterested //

** items not assessed at extubation and sternal recumbency; * items not assessed at extubation.