Table II. Distance from the bound nucleotide and Mg2+ to switch I residues.
Motor protein | Pα to | S596 | S597 | R598 |
---|---|---|---|---|
WT Kar3+N11 | 10.45 | 9.33 | 12.99 | |
Kar3 R598A | 10.58 | 9.16 | 12.84 | |
Kar3 E631A | 9.66 | 8.93 | 12.58 | |
Mg2+ to | S596 | S597 | R598 | |
WT Kar3+N11 | 6.23 | 5.64 | 9.08 | |
Kar3 R598A | 6.23 | 5.32 | 8.86 | |
Kar3 E631A | 5.52 | 4.95 | 8.50 |
Distances are in angstroms and were measured using the program O (Jones et al., 1991). Kar3 N650K is almost identical to wild-type Kar3+N11 and is therefore not shown.