Table I. Morphometric analysis of cardiomyocytes.
| Cell treatment | Area (µm2) | Major axis (µm) | Minor axis (µm) | Major/minor |
|---|---|---|---|---|
| Adβ-gal | 2167 ± 688 | 65 ± 11 | 42 ± 9.1 | 1.58 ± 0.39 |
| AdMEK1CA | 7146 ± 1560 | 134 ± 22* | 68 ± 12 | 2.38 ± 1.03** |
| AdMEK5DD | 3440 ± 1050 | 136 ± 37** | 34 ± 13** | 4.65 ± 2.28** |
| PE | 5745 ± 1560 | 110 ± 20 | 66 ± 12 | 1.71 ± 0.37 |
| PE + Adβ-gal | 6411 ± 2070 | 122 ± 23 | 66 ± 13 | 1.91 ± 0.44 |
| PE + AdMEK5KM | 4524 ± 1140* | 98 ± 15 | 59 ± 10 | 1.71 ± 0.35 |
| LIF | 3586 ± 1090 | 144 ± 36** | 33 ± 12** | 5.06 ± 2.35** |
| LIF + Adβ-gal | 3671 ± 874 | 148 ± 42** | 35 ± 14** | 5.37 ± 3.26** |
| LIF + AdMEK5KM | 4683 ± 889‡ | 101 ± 16‡ | 59 ± 10‡ | 1.78 ± 0.47‡ |
Neonatal rat cardiomyocytes were infected with virus and treated with PE (100 µM) or LIF (1000 U/ml) where indicated and as described in legends for Figures 2 and 3. Measurements were made using a computerized morphometric system. Thirty cells were examined for each adenovirus, and this was repeated for three independent experiments.
Values are means ± SD; *P <0.05 versus PE-treated (no virus); **P <0.001 versus PE-treated (no virus); ‡P <0.001 versus LIF-treated (no virus).