Table II. Plasmids used in this study.
| Plasmid | Description | Source or reference |
|---|---|---|
| pRS316 | low-copy URA3 vector | Sikorski and Hieter (1989) |
| YEplac181 | high-copy LEU2 vector | this study |
| pLPY-PRT1His | PRT1-His in YEplac181 | this study |
| pLPY-PRT1His-TIF34HA-TIF35Flag | PRT1-His, TIF34-HA, and TIF35-FLAG in YEplac181 | this study |
| YEplac195 | high-copy URA3 vector | this study |
| pLPY-NIP1 | NIP1 in Yeplac195 | this study |
| pLPY-NIP1-TIF32 | NIP1 and TIF32 in YEplac195 | this study |
| pLPY-TIF32 | TIF32 in Yeplac195 | this study |
| YCpLVHM-T | HCR1-Myc in TRP1 single-copy vector YCplac22 | Valášek et al. (2001) |
| pLPY201 | PRT1-His in pRS316 | this study |
| pLPY102 | PRT1 in low-copy LEU2 vector pRS315 | Greenberg et al. (1998) |
| pLPY202 | prt1-1-His in pRS316 | this study |