Fig. 3. The lag1Δlac1Δ cells cannot make PHS-C26OH. Two and a half OD of W303-1A (WT) and W303-1A lag1Δlac1Δ (2Δ) strains were pre-incubated with Aus, AbA or solvent for 10 min and were radiolabelled with 25 µCi of [³H]DHS at 30°C for 1 h. Lipid extracts were then O-deacylated with MMA or control incubated and analysed by TLC in solvent system 1.