Table I. lag1Δlac1Δ cells strongly up-regulate the biosynthesis of very long chain fatty acids^a.

	WT	2Δ	WT + HCl	2Δ + HCl
C14:1	0.235 ± 0.002	0.159 ± 0.061	0.127	0.046
C14:0	1.261 ± 0.159	0.567 ± 0.149	0.831	0.419
C16:1	23.59 ± 0.357	21.56 ± 0.387	25.31	25.07
C16:0	18.89 ± 0.283	11.91 ± 0.520	16.2	9.248
C18:1	38.73 ± 0.233	33.22 ± 0.548	44.74	43.65
C18:0	14.81 ± 1.381	24.12 ± 2.155	10.22	14.68
C20:1	0 ± 0.000	0 ± 0.000	0.332	0.544
C20:0	0.778 ± 0.230	1.53 ± 0.301	0.291	0.474
C22:1	0.479 ± 0.045	0.547 ± 0.086	0.369	0.966
C22:0	0.065 ± 0.065	0.288 ± 0.162	0.143	0.178
C24:1	0.438 ± 0.094	0.444 ± 0.107	0.271	0.691
C24:0	0.144 ± 0.144	0.447 ± 0.099	0.209	0.357
C26:1	0.038 ± 0.038	0.123 ± 0.123	0	0
C26:0	0.266 ± 1.434	4.886 ± 0.037	0.892	3.653

^aThe lipids of W303-1A and W303-1A lag1Δlac1Δ strains were methylated either directly (columns WT and 2Δ) or after strong acid hydrolysis (columns WT + HCl and 2Δ + HCl) and analysed by GC–MS as described in Materials and methods. C16, C18 and C26 fatty acids were used as standards. Numbers indicate the percentage of a given fatty acid species compared with the total of identified peaks in the gas chromatogram. With the BF₃ methylation procedure, we were not able to identify the hydroxylated fatty acids, since we had no appropriate standards and the conditions for their quantitative methylation had not been worked out. Thus, only 80% of the signals of the GC profile could be accounted for. Standard deviations represent variations obtained in two independent experiments. The same results as shown in columns WT and 2Δ were obtained when lipid extracts were treated with MMA before being methylated with methanolic BF₃, indicating that the release of fatty acids from glycerophospholipids was complete (not shown).