Table 2.
System suitability parameters for PHE, DOX and DOX DEG in the proposed methods.
| Parameters | HPLC method | HPTLC method | Reference value | ||||
|---|---|---|---|---|---|---|---|
| PHE | DOX | DOX DEG | DOX | PHE | DOX DEG | ||
| Retention time (Rt) | 2.79 min | 5.41 min | 6.45 min | - | - | ||
| Retardation factor (Rf) | - | 0.31 | 0.64 | 0.78 | - | ||
| Resolution (Rs) | - | 7.68 | 6.03 | - | 7.42 | 3.16 | > 1.5 |
| Capacity factor (K) | 1.79 | 4.41 | 5.46 | 2.22 | 0.56 | 0.28 | 0–10 |
| Tailing factor (T) | 0.95 | 1.02 | 0.96 | 0.95 | 1.17 | 1.07 | ≤ 2 |
| Selectivity (α) | - | 2.46 | 1.23 | - | 3.96 | 2 | > 1 |
| Number of theoretical Plate (N) | 2483 | 17,039 | 22,390 | - | > 2000 | ||
| Height equivalent to theoretical plate (HETP) | 0.10068 | 0.07096 | 0.04577 | - | The smaller the value, the higher the column efficiency | ||