Table 3.

Robustness assessment of the proposed methods for determination of PHE and DOX.

Parameters		HPLC method						HPTLC method
		Change in ratio of ethanol (%)		Mobile phase flow rate (mL/min)		Detector wavelength (nm)		Change in ratio of methylene chloride (%)		Saturation time (min)
		29	31	0.9	1.1	260	261	2	3	25	35
Measured Rt	PHE	2.77	2.81	2.80	2.77	2.78	2.81	-
	DOX	5.42	5.39	5.4	5.38	5.4	5.43
Measured Rf	PHE	-						0.29	0.32	0.30	0.29
	DOX							0.64	0.63	0.64	0.65
Measured K	PHE	1.80	1.77	1.79	1.81	1.8	1.78	2.22	2.23	2.21	2.23
	DOX	4.42	4.40	4.41	4.39	4.41	4.39	0.56	0.55	0.55	0.57
Measured T	PHE	0.95	0.94	0.96	0.95	0.93	0.94	0.95	0.96	0.94	0.95
	DOX	1.02	1.01	1.02	1	1.01	1.02	1.16	1.15	1.15	1.16