Fig. 3. Wild-type JIP1 but not JIP(307–711) inhibits DLK dimerization. COS 7 cells were co-transfected with HA-DLK (0.1 µg), Flag-DLK (0.1 µg) and increasing quantities of Myc-JIP1 plasmids as indicated. (A) Flag-DLK complexes were immunoprecipitated from cell lysates using anti-Flag antibody, separated by SDS–PAGE and immunoblotted with either anti-HA or anti-Myc antibody. Correspond ing cell lysates were immunoblotted to evaluate the relative expression of Myc-JIP, HA-DLK and Flag-DLK proteins. (B) Cells were co-transfected with HA-DLK (0.1 µg), Flag-DLK (0.1 µg) and the indicated quantity of Myc-JIP or Myc-JIP(307–711) plasmids; samples were analyzed as above.