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Fig. 4. (A) Representative flow cytometry scatter plots of MEFs nuclei 18 h after irradiation (5 Gy), plotted as increasing fluorescence of propidium iodide (PI; x-axis) versus increasing FITC fluorescence obtained with an anti-BrdU–FITC-conjugated antibody (y-axis). Gating for S-phase cells is shown in the panel from unirradiated wild-type mice (upper left). (B) Graph representing the mean ± SD change in the percentage of S-phase cells after irradiation (n = 3–6).
