Table 4.

Features of PCR primers

Name	Orientation	Sequence (5′ 3′)	Amplicon size (nucleotides)
A1	Forward	TGCTTGAGCTTGGGGTACTTT	580
A2	Reverse	GTTACAGGGGTGTAGTGGGC
A1	Forward	TGCTTGAGCTTGGGGTACTTT	422
A3	Reverse	CTGCTGGGGTGGGAAATCTG

See Fig. 2 for details about primer pairs usage