Fig. 2. Complementation of mouse C3G deficiency by the human C3G transgene. (A) Schematic illustration of an expression plasmid of the human C3G transgene. The human C3G gene, which is sandwiched with two loxP recombination sites, is placed downstream of the chicken β-actin promoter and the CMV immediate early enhancer. SalI and BamHI sites, which were used to isolate the transgene expression unit, are indicated by arrows. (B) Southern blot analysis of transgenic mice crossed with C3G knockout mice. The genotype of mouse C3G and the presence of the human C3G transgene are indicated at the top.