Fig. 1. Equilibrium sedimentation analysis of recombinant REGγ. Data collected at different concentrations of REGγ were fit simultaneously to a single species model. The lower panel shows the representative experimental data (circles, 110 µg/ml; diamonds, 55 µg/ml) of wild-type REGγ and the calculated curve fits (continuous lines) for a single species model. The resulting fit was good as shown by the randomly distributed residuals (upper panel). The returned molecular weight for REGγ is 214 ± 8 kDa.