Table II. Summary of immunohistochemistry data.
| E13.5 # | Genotype | Sex | % Rb–/– | TUNEL | Ect. BrdU | PH3 |
|---|---|---|---|---|---|---|
| 1 | chimera | m | nd | 53.5 | nd | 30 |
| 2 | chimera | f | 36.3 | 58.5 | nd | nd |
| 3 | chimera | f | 67.5 | 182 | 586.5 | 55 |
| 4 | chimera | f | 72.5 | 174.5 | 468.5 | 67 |
| 5 | chimera | m | nd | 178.5 | 652.5 | 46.3 |
| 6 | chimera | m | nd | 100 | 548 | 43 |
| 7 | chimera | f | 63.2 | 63.5 | nd | 51.3 |
| 8 | chimera | f | 39.8 | 28 | 320 | 48.5 |
| Average | 55.9 | 111.1 | 535.4 | 48.7 | ||
| SD | 16.6 | 64 | 127.8 | 11.3 | ||
| 9 | Rb–/– | nd | 100 | nd | nd | 154 |
| 10 | Rb–/– | nd | 100 | nd | nd | 148.5 |
| 11 | Rb–/– | nd | 100 | 526 | 585 | 141.5 |
| 12 | Rb–/– | nd | 100 | 492 | 484 | 183 |
| 13 | Rb–/– | nd | 100 | 444.5 | nd | nd |
| 14 | Rb–/– | nd | 100 | nd | 404 | nd |
| 15 | Rb–/– | nd | 100 | 247 | 277 | nd |
| Average | 427.4 | 437.5 | 156.7 | |||
| SD | 124.8 | 130.1 | 18.2 | |||
| 16 | wt | nd | 0 | 3.5 | 21 | 62 |
| 17 | wt | nd | 0 | 1.5 | 30 | 52.3 |
| 18 | wt | nd | 0 | 6 | 54 | 28.7 |
| 19 | wt | nd | 0 | nd | nd | 30.6 |
| 20 | wt | nd | 0 | 3 | nd | nd |
| 21 | wt | nd | 0 | 4.5 | nd | nd |
| 22 | wt | nd | 0 | 2.5 | nd | nd |
| Average | 3.5 | 31.7 | 43.4 | |||
| SD | 1.6 | 17.1 | 16.4 | |||
| wt/ch t-test | 0.0023 | 0.0007 | 0.5364 | |||
| Rb/ch t-test |
|
|
|
0.0001 |
0.3989 |
6.2 × 10–7 |
| E15.5 # |
Genotype |
Sex |
% Rb–/– |
TUNEL |
||
| 23 | chimera | f | nd | 20 | ||
| 24 | chimera | f | 31.3 | 19 | ||
| 25 | chimera | f | 70.9 | 28.5 | ||
| 26 | chimera | m | nd | 73 | ||
| 27 | chimera | m | nd | 108 | ||
| 28 | chimera | m | nd | 34 | ||
| 29 | chimera | m | nd | 57.5 | ||
| 30 | chimera | m | nd | 29 | ||
| 31 | chimera | f | nd | 36 | ||
| 32 | chimera | f | 58.7 | 46.5 | ||
| Average | 53.6 | 45.1 | ||||
| SD | 20.3 | 27.8 | ||||
| 33 | wt | nd | 0 | 10 | ||
| 34 | wt | nd | 0 | 18.5 | ||
| 35 | wt | nd | 0 | 17 | ||
| 36 | wt | nd | 0 | 12 | ||
| Average | 14.4 | |||||
| SD | 4 | |||||
| wt/ch t-test |
|
|
|
0.0521 |
||
| Neonate # |
Genotype |
Sex |
% Rb–/– |
TUNEL |
||
| 37 | chimera | nd | nd | 225 | ||
| 38 | chimera | nd | nd | 183 | ||
| 39 | chimera | m | nd | 377.5 | ||
| 40 | chimera | f | nd | 275.3 | ||
| 41 | chimera | m | nd | 485 | ||
| 42 | chimera | m | nd | 275 | ||
| Average | 303.5 | |||||
| SD | 110.2 | |||||
| 43 | wt | nd | nd | 133.5 | ||
| 44 | wt | nd | nd | 131.5 | ||
| Average | 132.5 | |||||
| SD | 1.4 | |||||
| wt/ch t-test |
|
|
|
0.0825 |
||
| Adult # |
Genotype |
Sex |
% Rb–/– |
TUNEL |
||
| 45 | chimera | m | 60 | 12.5 | ||
| 46 | chimera | f | 90 | 12.5 | ||
| 47 | chimera | m | 80 | 27.5 | ||
| 48 | chimera | m | 15 | 16.5 | ||
| Average | 61.2 | 17.2 | ||||
| SD | 33.3 | 7.1 | ||||
| 49 | wt | m | 0 | 15 | ||
| 50 | wt | f | 0 | 7.5 | ||
| Average | 11.2 | |||||
| SD | 5.3 | |||||
| wt/ch t-test | 0.2849 |
Ect. BrdU, ectopic BrdU incorporation; PH3, phospho-histone 3; f, female; m, male; nd, not determined; Rb/ch t-test, two-tailed T-test for the difference between Rb–/– and chimeric animals; wt/ch t-test, p-value for two-tailed T-test for the difference between wild-type and chimeric animals. For other abbreviations see Table I.