Skip to main content
NCBI home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1987 Jan;51(1):50–55.

Malignant hyperthermia susceptibility: cardiac histomorphometry of dogs and young and market-weight swine.

P J O'Brien, T F Fletcher, A L Metz, H J Kurtz, B K Reed, W E Rempel, E G Clark, C F Louis
PMCID: PMC1255273  PMID: 3567752

Abstract

The defect causing malignant hyperthermia has been proposed to involve cardiac as well as skeletal muscle. We tested the hypothesis that histomorphometric parameters for ventricular wall from malignant hyperthermia-susceptible swine and dogs were abnormal. Hearts were obtained from: mature dogs, age- and weight-matched young swine (89 +/- 15 days, 30 +/- 3 kg); and market-weight swine (102 +/- 10 kg). Using light microscopy, estimates were made for muscle nuclear dimensions and the volume-fraction of nuclei, sarcoplasm, blood vessels, and interstitial space. Cardiac maturation in both MH and normal swine was accompanied by decreased myocyte volume-fraction due to decreased nuclear volume-fraction and increased interstitial space volume-fraction. Sarcoplasm and vasculature volume-fraction were unchanged after maturation. Nuclear volume-fraction was slightly greater (p less than 0.05) in the right ventricle than the left for malignant hyperthermia and normal swine. Myocyte nuclear dimensions were generally similar among animals. Dogs and the oldest group of swine were not significantly different. Myocytes of all swine contained multiple nuclei, closely spaced in rows of 2 to 12. In contrast, most myocytes of mature dogs apparently contained one or two nuclei. Histomorphometric values were not significantly different between normal and malignant hyperthermia young swine and dogs. However, within the market-weight swine, volume-fraction for malignant hyperthermia myocytes and myocyte nuclei was decreased and interstitial space was increased compared to normal.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
50

Images in this article

52Fig. 1.
on p.52

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler C. P., Friedburg H. Myocardial DNA content, ploidy level and cell number in geriatric hearts: post-mortem examinations of human myocardium in old age. J Mol Cell Cardiol. 1986 Jan;18(1):39–53. doi: 10.1016/s0022-2828(86)80981-6. [DOI] [PubMed] [Google Scholar]
  2. Anversa P., Loud A. V., Giacomelli F., Wiener J. Absolute morphometric study of myocardial hypertrophy in experimental hypertension. II. Ultrastructure of myocytes and interstitium. Lab Invest. 1978 May;38(5):597–609. [PubMed] [Google Scholar]
  3. Astorri E., Bolognesi R., Colla B., Chizzola A., Visioli O. Left ventricular hypertrophy: a cytometric study on 42 human hearts. J Mol Cell Cardiol. 1977 Sep;9(9):763–775. doi: 10.1016/s0022-2828(77)80021-7. [DOI] [PubMed] [Google Scholar]
  4. Baroldi G., Falzi G., Lampertico P. The nuclear patterns of the cardiac muscle fiber. Cardiologia. 1967;51(2):109–123. doi: 10.1159/000165856. [DOI] [PubMed] [Google Scholar]
  5. Bishop S. P., Hine P. Carciac muscle cytoplasmic and nuclear development during canine neonatal growth. Recent Adv Stud Cardiac Struct Metab. 1975;8:77–98. [PubMed] [Google Scholar]
  6. Elizondo G., Addis P. B., Rempel W. E., Madero C., Martin F. B., Anderson D. B., Marple D. N. Stress response and muscle properties in pietrain (P), Minnesota no 1 (M) and PxM pigs. J Anim Sci. 1976 Nov;43(5):1004–1014. doi: 10.2527/jas1976.4351004x. [DOI] [PubMed] [Google Scholar]
  7. Gronert G. A. Malignant hyperthermia. Anesthesiology. 1980 Nov;53(5):395–423. doi: 10.1097/00000542-198011000-00007. [DOI] [PubMed] [Google Scholar]
  8. Huckell V. F., Staniloff H. M., Britt B. A., Morch J. E. Electrocardiographic abnormalities associated with malignant hyperthermia susceptibility. J Electrocardiol. 1982 Apr;15(2):137–141. doi: 10.1016/s0022-0736(82)80007-1. [DOI] [PubMed] [Google Scholar]
  9. Huckell V. F., Staniloff H. M., Britt B. A., Waxman M. B., Morch J. E. Cardiac manifestations of malignant hyperthermia susceptibility. Circulation. 1978 Nov;58(5):916–925. doi: 10.1161/01.cir.58.5.916. [DOI] [PubMed] [Google Scholar]
  10. Hwang P. T., McGrath C. J., Addis P. B., Rempel W. E., Thompson E. W., Antonik A. Blood creatine kinase as a predictor of the porcine stress syndrome. J Anim Sci. 1978 Sep;47(3):630–633. doi: 10.2527/jas1978.473630x. [DOI] [PubMed] [Google Scholar]
  11. Isaacs H., Frere G., Mitchell J. Histological, histochemical and ultramicroscopic findings in muscle biopsies from carriers of the trait for malignant hyperpyrexia. Br J Anaesth. 1973 Aug;45(8):860–868. doi: 10.1093/bja/45.8.860. [DOI] [PubMed] [Google Scholar]
  12. Johansson G., Olsson K., Häggendal J., Jönsson L., Thorén-Tolling K. Effect of stress on myocardial cells and blood levels of catecholamines in normal and amygdalectomized pigs. Can J Comp Med. 1982 Apr;46(2):176–182. [PMC free article] [PubMed] [Google Scholar]
  13. Jönsson L., Johansson G., Lannek N., Lindberg P. Intramural blood supply of porcine heart. A postmortem angiographic study. Anat Rec. 1974 Mar;178(3):647–656. doi: 10.1002/ar.1091780312. [DOI] [PubMed] [Google Scholar]
  14. Langes K., Arnold G., Pfitzer P. Postnatal DNA-syntheses and mitoses in hearts of dwarf pigs. J Mol Cell Cardiol. 1983 Dec;15(12):831–844. doi: 10.1016/0022-2828(83)90345-0. [DOI] [PubMed] [Google Scholar]
  15. Mambo N. C., Silver M. D., McLaughlin P. R., Huckell V. F., McEwan P. M., Britt B. A., Morch J. E. Malignant hyperthermia susceptibility. A light and electron microscopic study of endomyocardial biopsy specimens from nine patients. Hum Pathol. 1980 Jul;11(4):381–388. doi: 10.1016/s0046-8177(80)80036-0. [DOI] [PubMed] [Google Scholar]
  16. O'Brien P. J., Forsyth G. W., Olexson D. W., Thatte H. S., Addis P. B. Canine malignant hyperthermia susceptibility: erythrocytic defects--osmotic fragility, glucose-6-phosphate dehydrogenase deficiency and abnormal Ca2+ homeostasis. Can J Comp Med. 1984 Oct;48(4):381–389. [PMC free article] [PubMed] [Google Scholar]
  17. O'Brien P. J., Rooney M. T., Reik T. R., Thatte H. S., Rempel W. E., Addis P. B., Louis C. F. Porcine malignant hyperthermia susceptibility: erythrocytic osmotic fragility. Am J Vet Res. 1985 Jul;46(7):1451–1456. [PubMed] [Google Scholar]
  18. O'brien P. J., Cribb P. H., White R. J., Olfert E. D., Steiss J. E. Canine malignant hyperthermia: diagnosis of susceptibility in a breeding colony. Can Vet J. 1983 Jun;24(6):172–177. [PMC free article] [PubMed] [Google Scholar]
  19. Sandritter W., Adler C. P. Numerical hyperplasia in human heart hypertrophy. Experientia. 1971 Dec 15;27(12):1435–1437. doi: 10.1007/BF02154274. [DOI] [PubMed] [Google Scholar]
  20. Webb A. J. The halothane test: a practical method of eliminating porcine stress syndrome. Vet Rec. 1980 May 3;106(18-20):410–412. doi: 10.1136/vr.106.18-20.410. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES