Fig. 1. Immunostaining of Pin1 in human breast cancer. Sections from paraffin-embedded tissues were subjected to an antigen retrieval treatment, followed by immunostaining with anti-Pin1 antibodies. Non-cancerous tissues (A and B; normal breast with mild fibrocystic changes) show weak, but detectable, Pin1 staining, while invasive ductal carcinomas (C and D) or ductal carcinoma in situ (E) show intense Pin1 staining. To show the specificity of Pin1 antibodies, Pin1-specific antibodies were first depleted using GST–Pin1 beads and then used to stain the breast sections (F).