Fig. 9. Generation of ALEX upon transfection of the XLαs cDNA in PC12 cells. PC12 cells were either not transfected (wt) or transfected with the full-length XLαs cDNA (Kehlenbach et al., 1994) (XLαs) or the ALEX cDNA fragment derived from it (ALEX). (A) Total cell membranes (280 µg protein) were analyzed by immunoblotting using the anti-XL antibody. (B) The Triton X-100-insoluble material obtained from these membranes was analyzed by immunoblotting using the anti-ALEX antibody. (C) Quantification of the XLαs band of (A) and the ALEX band of (B) by densitometric scanning. The values obtained for the untransfected cells were set arbitrarily to 1 and the other values expressed relative to this.