Fig. 6. Silica gel 60 HPTLC analysis of the predominant promastigote glycolipids of L.mexicana in WT (lane 1), ΔGDPMP (lane 2) and ΔGDPMP + cRIBLmxGDPMP (lane 3) parasites. (A) Total lipids from 2 × 10⁸ promastigotes visualized by orcinol/H₂SO₄ spraying. (B) Fluorography of total lipids from 2.5 × 10⁷ [³H]Man-labeled promastigotes (∼100 000 c.p.m.). (C) Fluorography of total lipids from 5 × 10⁶ [³H]GlcNH₂-labeled promastigotes (∼100 000 c.p.m.). (D) Fluorography of total lipids from 5 × 10⁶ [³H]myo-inositol-labeled promastigotes (∼100 000 c.p.m.). The positions of the abundant L.mexicana GIPLs (McConville et al., 1993) are indicated by the bars, and the start and front of the TLCs are marked by S and F, respectively. Asterisks mark new [³H]GlcNH₂-labeled compounds accumulating in the ΔGDPMP mutant.