Fig. 7. Analysis of macrophage and mouse infections by L.mexicana WT, ΔGDPMP and LmxGDPMP gene addback mutants. (A and B) Infection of peritoneal macrophages by L.mexicana WT, ΔGDPMP, ΔGDPMP + pXLmxGDPMP and ΔGDPMP + cRIBLmxGDPMP. Peritoneal macrophages were infected at a ratio of two stationary phase promastigotes per cell. The percentage of infected macrophages (sample size 300) was counted 6 days (A) or at day 1, 2, 3, 4 and 7 (B) after the infection. The standard errors of duplicate experiments are indicated. In in vivo infection experiments, Balb/c mice were challenged with 1 × 10⁷ L.mexicana promastigotes in the right hind footpad. The swelling caused by L.mexicana WT, ΔGDPMP and ΔGDPMP + pXLmxGDPMP (C), and ΔGDPMP + cRIBLmxGDPMP (D) was recorded. The infection experiments were performed in quadruplicates and the standard errors are indicated.