Table II. Apparent kinetic parameters of the diferent CAK complexes with the CTD and CDK2–cyclin A substrates.

Parameter	CTD			CDK2D145N–cyclin A
	Dimer	Trimer	P-Trimer	Dimer	Trimer	P-Trimer
Km (µM)	7.5 ± 2.5	3.8 ± 1.6	4.3 ± 1.4	0.26 ± 0.08	0.39 ± 0.07	0.23 ± 0.05
Vmax (pmol Pi/min)	1.6 ± 0.3	1.6 ± 0.2	26.6 ± 3.3	0.094 ± 0.010	0.054 ± 0.010	0.19 ± 0.02
kcat (/s)	0.23	0.22	3.69	0.013	0.008	0.026
kcat/Km	0.03	0.06	0.86	0.04	0.02	0.11