Fig. 1. Nuclear accumulation of Rpl25p–eGFP in rix7-1 cells. (A) Growth curve of rix7-1 and isogenic wild-type cells at 37°C. (B) rix7-1 and isogenic wild-type cells expressing Rpl25p–eGFP were grown at 23°C, before shift for 5 h to 35°C and inspection in the fluorescence microscope. The arrowhead points to nuclear staining and the triangle to nucleolar staining.