Fig. 4. rix7-1 is impaired in 60S subunit biogenesis. Polysomal profiles (OD_{260 nm}) after sucrose density gradient centrifugation derived from the rix7-1 mutant and wild-type strain grown at permissive temperature (23°C) and 4 h after shift to restrictive temperature (37°C) under low salt (100 mM KCl) (A) and high salt (0.8 M KCl) conditions (B). The positions of 40S, 60S and 80S ribosomal particles, polysomes and ‘half-mers’ are indicated.