Fig. 6. Disruption of the subterminal contacts using a short transposon end fragment. The standard gel shift assay was performed using the standard BglII–SalI transposon end fragment or a short fragment with only 52 bp of transposon DNA. Assignment of the bands is as in Figure 1B. With the standard fragment, the quantity of bDEB appears greater than the cleaved flanking DNA because the SalI site is labeled more efficiently than the BglII site. The short end is only labeled on the flanking DNA because the opposite end is blunt.