Figure 5.

RP-HPLC separation of detritylated 5mer Oxa-ODN (5′-GCOAT-3′) purified by Poly-Pak cartridge (a), and DNA monomer, dCyd, dGuo, dThd, dOxo and dAdo formed by the digestion of with nuclease P1 and alkaline phosphatase (b). HPLC condition: mobile phase; 100 mM TEAA solution (pH 7.0) with a gradient of CH₃CN [for (a), 7.6% (0 min)∼ 14% (40 min) (please see Materials and Methods) and for (b), 0% (0 min)∼20% (20 min)], with flow rate; 1 ml/min, column; Ultron VX-ODS columns [150 × 4.6 mm, 5 µm; Shinwa Co. (Kyoto, Japan)].