Table 3.
Sequence | Position | Coupling yield | ||||
---|---|---|---|---|---|---|
e d c b a | a∼ba | b∼c (%) | c∼d (%) | d∼ea | a∼ea | |
COG-18 | 5′-TCT CTT CCO GCC TAC GAT-3′ | 96.96% ± 2.44% | 93.39 | 97.82 | 96.92 ± 2.59% | 96.70 ± 2.52% |
TOA-18 | 5′-TCT CTT CTO ACC TAC GAT-3′ | 96.58% ± 2.23% | 96.28 | 96.60 | 97.64 ± 1.19% | 97.02 ± 1.79% |
GOC-18 | 5′-TCT CTT CGO CCC TAC GAT-3′ | 97.09% ± 2.30% | 98.06 | 97.28 | 96.59 ± 1.26% | 97.16 ± 2.23% |
AOT-18 | 5′-TCT CTT CAO TCC TAC GAT-3′ | 97.28% ± 1.86% | 99.09 | 97.60 | 97.42 ± 1.32% | 97.40 ± 1.86% |
aAverage step-wise coupling yield.