Table 4.

Melting temperatures and thermodynamic parameters of DNA duplexes containing O:N and G:C base-pairing

Type	Sequence	X	N	Tma (°C)	ΔTmb(°C)	−ΔH0 (kcal mol−1)	−ΔS0 (cal mol−1 K−1)	$-\Delta G_{37}^0$c (kcal mol−1)	$-\Delta \Delta G_{37}^0$d (kcal mol−1)
		O	C	64.49	−6.83	130.396	371	15.293	2.643
-CXG-	5′-TCT CTT CCX GCC TAC GAT-3′	O	T	61.15	−10.17	126.664	365	13.494	4.442
-GNC-	3′-AGA GAA GGN CGG ATG CTA-5′	O	G	62.57	−8.75	105.994	303	11.889	6.047
		O	A	62.68	−8.64	113.296	323	13.073	4.863
		G	C	71.32	0	135.595	379	17.936	0
		O	C	56.80	−9.92	130.574	381	12.332	3.648
-TXA-	5′-TCT CTT CTX ACC TAC GAT-3′	O	T	55.37	−11.35	124.935	367	11.118	4.862
-ANT-	3′-AGA GAA GAN TGG ATG CTA-5′	O	G	53.31	−13.41	115.422	339	10.191	5.789
		O	A	54.51	−12.21	117.079	343	10.563	5.417
		G	C	66.72	0	132.679	376	15.980	0

^aT_m was measured in case that the total concentrations of ODNs is 4 µM.

^bΔT_m was calculated by T_m−T_m(G:C).

^c$\Delta G_{37}^0$ was calculated by ΔH⁰ − (310.15 × ΔS⁰).

^d$\Delta \Delta G_{37}^0$ was calculated by $\Delta G_{37}^0-\Delta G_{37}^0$ (G:C).