Fig. 7. Characterization of HMIT protein. (A) Left: immuno precipitation of HMIT expressed in oocytes using anti-Flag antibodies; (+) injected oocytes, (–) non-injected oocytes. Right: immunoprecipitation of HMIT synthesized from synthetic RNA in a rabbit reticulocyte lysate in the presence (+) or absence (–) of canine pancreas microsomal membranes. (B) Western blot analysis of HMIT present in membrane proteins from transiently transfected (+) or mock-transfected (–) HEK293T, or in membrane preparations from adultrat brain (Brain), using an anti-HMIT rabbit polyclonal antibody. Membrane proteins were subjected (+) or not (–) to PNGase F treatment prior to electrophoretic separation.