Fig. 4. Treatment with the m⁷GpppG cap analog disrupts the subnuclear distribution of eIF4E, PML and SP100. Cells were treated with buffer, GpppG or m⁷GpppG as indicated. U937 cells (A–C) and K562 cells (D–F) were stained for PML (dark blue) and eIF4E (green). The overlay is aqua. (G–I) K562 cells were stained for the PML partner protein Sp100. The objective is 100×. Scale bars = 10 µM. Confocal micrographs represent single sections through the plane of the cells.