Fig. 5. Treatment with the m⁷GpppG cap analog does not alter the subnuclear distribution of Sm speckles or nucleoli. Cells were treated with buffer, GpppG or m⁷GpppG as indicated. U937 and K562 cells were stained for Sm and nucleolin, respectively. The objective is 100×. Scale bars = 5 µM. Confocal micrographs represent single sections through the plane of the cells.