Fig. 3. Accumulated mutation density in 100 bp intervals. Details of how the mutation density is calculated are included in Materials and methods. The black line represents pooled mutation density. The grey lines are fitted curves to the experimental data. The insets show the equations and R² values for the exponential fits. (A) Pooled light chains. N = 1 corresponds to position 239 from the initiation Met in the Lκ light chain. (B) Top curves correspond to pooled JH2, three and four rearrangements, while the bottom curves are derived from JH₂ and JH₄ rearrangements. N = 1 corresponds to position 330, 713 and 1284 of JH₂, JH₃ and JH₄, respectively, as in Figure 1A. (C) Pooled mutation density for heavy and light chains. N = 1 corresponds to position 531 of Lκ for the light chains and the same as (B) for heavy chains.