Fig. 4. Cdt1 potentiates re-replication induced by Cdc18. (A) FACS analysis of cdc25-22 cells and cdc25-22 nmt1-cdc18+ cells expressing Cdt1 on a pREP4 plasmid, shifted to 37°C in the absence of thiamine. (B) DAPI staining of cells expressing either Cdc18 alone or both Cdc18 and Cdt1 at 0 and 10 h after the shift to 37°C in the absence of thiamine. (C) FACS analysis of cells expressing both Cdc18 and Cdt1 shifted to 37°C in the absence of thiamine and treated with 100 µg/ml cycloheximide at 6 h.