Fig. 4. Subcellular localization of Ara6–GFP and GFP–Ara7. ARA6 and ARA7 were tagged with GFP and expressed transiently in the protoplasts of Arabidopsis suspension cultured cells. (A and B) An Arabidopsis cell expressing GFP. (C–G) Ara6–GFP was localized on motile dots and ring-like organelles (arrowheads). (H and I) GFP–Ara7 was localized predominantly on the motile dotted organelles. (J and K) Protoplasts not treated with the plasmid showed no fluorescence. (A, C, E, H and J) Confocal images. (B, D, F, I and K) Nomarski images. (G) Projection of serial confocal images taken at 0.25 mm intervals. (B–K) Same scale as (A). Bar, 10 µm. (L) Distribution of Ara6 examined by immunofluorescence analysis. Protoplasts, some of which are expressing ARA6–GFP, were fixed and stained with the anti-Ara6 antibody. Asterisks indicate untransformed cells. Bar, 10 µm.