Fig. 9. (A–D) The N-terminal region of Ara6 is not sufficient for endosomal localization. A fusion of the N-terminal 34 amino acids of Ara6 with GFP (N34–GFP) was expressed transiently in Arabidopsis suspension cultured cells. (E–H) Nucleotide binding is required for the endosomal localization of Ara6. The nucleotide-free mutant of Ara6 tagged by GFP, Ara6N147I–GFP, was expressed transiently in Arabidopsis suspension cultured cells. (A, C, E and G) Confocal images. (B, D, F and H) Nomarski images. Bar, 10 µm.